Technical Note : Function-Based Discovery of Anti-HER2 Antibodies: From Mouse Immunization to Hit Sequences 

Function-Based Discovery of Anti-HER2 Antibodies: From Mouse Immunization to Hit Sequences

• Mouse immunization against recombinant HER2 antigen and response monitoring by ELISA
• Plasma cell enrichment and same-day, single-cell identification of hits onto the Beacon® optofluidic platform (Bruker)
• Real-time dual-channel assay: simultaneous antibody secretion (AF488) and antigen binding (HER2–AF647) at single-cell resolution
• Automated export of selected antibody-secreting cells, BCR genes recovery by RT-PCR for sequence analysis and downstream production

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