Functional
Characterization
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Custom Advanced Characterization For any target, toward any goal
At MImAbs, we design and tailor functional, biochemical, and biophysical assays to precisely characterize molecular mechanisms and biological activities. Our deep expertise guarantees robust, quantitative, and reproducible data across a wide range of targets and therapeutic modalities.
Biochemical profiling
Analyse antibody binding, affinity and cross-reactivity to ensure reliable selection and performance.
Functional confidence
Demonstrate antibody internalization & cytotoxic potential or immune activation with cell-based assays.
De-risk your pipeline
Our experts help you identify potential liabilities early to support smooth pre-clinical and clinical progression.
Trusted Discovery & Validation of Lead Antibodies
Advanced Characterization Techniques
Leverage our expertise in flow cytometry, BLI (Octet) and NanoDSF to gain reliable and insightful molecular profiling data.
Development of Custom Bioassays
We design and tailor, robust assays for your project needs.
Diverse Cell Models
Use our extensive cell bank – including primary cells, tumor cell lines, custom transfectants, and PDX-derived cell lines – to generate predictive results and minimize translational risk.
- Affinity and kinetics measurements: determine KD, kon/koff, and EC50 by Octet and flow cytometry.
- Competition assays and epitope binning: identify binding mechanisms and compare multiple antibodies.
- Cross-reactivity: evaluation on 2–5 cell lines by flow cytometry.
Evaluate antibody-driven immune responses with customizable, cell-based functional assays
Our ADCC, CDC, and ADCP assays assess antibody-mediated effector functions. ADCC measures antibody-dependent cellular cytotoxicity, CDC evaluates complement-dependent cytotoxicity, and ADCP quantifies antibody-dependent cellular phagocytosis. Using robust, standardized protocols, we deliver reliable, reproducible data to support pre-clinical development.
Pre-CMC Analysis for Antibodies and ADCs
We support the selection of antibodies and ADCs with high developability and manufacturability through pre-CMC derisking studies. These studies assess performance under thermal and mechanical and pH stress conditions, and include:
- Nano Differential Scanning Fluorometry (nanoDSF) – Evaluates thermal stability (Tm), aggregation points, and preformulation readiness.
- Analytical Size Exclusion Chromatography (SEC-UPLC) – Accurately quantifies aggregate content for robust biophysical characterization to ensure antibody and ADC quality.
- Mass Spectrometry (LC-MS) – Provides exact mass determination and monitors DAR stability for ADCs.
- Hydrophobic Interaction Chromatography (HIC) – Compare ADC hydrophobicity with parental antibody for informed linker-payload selection.
Binding Properties – Measures EC50 by flow cytometry (cell-based assay) and affinity by Octet (BLI), ensuring functional integrity.
